HIMA F7553
HIMA F7553 - Image 2
HIMA F7553 - Image 3
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HIMA F7553

Product details:

Origin: Germany

Brand name: HIMA

Model: F7553

Terms of payment and shipment:

Minimum order quantity: 1 piece

Packaging details: new factory and original factory, separate packaging

Delivery time: 2-3 working days

Payment method: telegraphic transfer, Western Union remittance

Supply capacity: 10+pieces

This assay is intended for use with samples such as Tissue

Homogenates. The sample collection protocols below have been

provided for your reference.

Breast Milk – Centrifuge samples for 20 minutes at 1000 × g to remove

particulates. Collect the supernatant for assaying. Store un-diluted

samples at -20°C or below. Avoid repeated freeze-thaw cycles.

Cell Lysates – Collect and pellet the cells by centrifugation and remove

the supernatant. Wash the cells 3 times with PBS* then resuspend in

PBS*. Lyse the cells by ultrasonication 4 times. Alternatively freeze the

cells to -20°C and thaw to room temperature 3 times. Centrifuge at

one thousand and five hundred × g for 10 minutes at 2 – 8°C to remove cellular debris. Collect the

supernatant for assaying. Store un-diluted samples at -20°C or below.

Avoid repeated freeze-thaw cycles.

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Description

HIMA F7553Upon receipt the kit should be stored at 4°C if intended for use within
24 hours. Otherwise the Assay Plate, Standard, Detection Reagent A,
and Detection Reagent B should be stored at -20°C. Avoid repeated
freeze-thaw cycles. Store all other kit components at 4°C. The
Substrate should never be frozen. Once individual reagents are opened
it is recommended that the kit be used within 1 month. Unused Strip
Plate wells should be stored at -20°C in a sealed bag containing
desiccant in order to minimize exposure to moisture. Do not use the kit
beyond its expiration date.
OTHER REQUIRED SUPPLIES
 Microplate reader with 450nm wavelength filter
 High-precision pipette and sterile pipette tips
 Eppendorf tubes
 37°C incubator
 Deionized or distilled water
 Absorbent paper
The following is an example of how to layout a study. A dilution series
of the positive control Standard should be run in duplicate or triplicate,
with the last well in each series being the negative control blank.
Samples should also be run in duplicate or triplicate. Unknown samples
should be run as a dilution series in order to identify the optimal
dilution that produces an OD reading within the OD range of the
positive control Standard dilution series.

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